IISAAC
IISAAC (Interactions by In Situ Affinity-tagging And Crosslinking, also called APEX-RIP) is a universal method for mapping the sub-cellular localization of RNA, transcriptome-wide. IISAAC uses in situ proximity-restricted biotinylation to isolate RNAs that reside in or near a target sub-cellular compartment, including structures that are recalcitrant to conventional biochemical fractionation. This approach can be applied in parallel to proteomic, transcriptomic and genomic analyses, allowing us to comprehensively catalog the macromolecular composition (proteins, RNAs, DNA loci) of target sub-cellular regions at unprecedented breadth.